1) Blood collection tubes, syringes and plasma containers shall be made of silicified glass or plastic products.
2) Do not pat your forearm before blood collection.
3) Blood collection should be smooth, and the second tube should be used for hem agglutination examination.
4) The ratio of sodium citrate to blood is 1:9 (pay attention to HCT). Gently reverse and mix well.
5) The specimen should be fresh (2 hours at room temperature), and the plasma should be stored at (- 70 ° C) when refrigerated. Melt rapidly at 37 ° C before the experiment.
6) Subject Status: physiological changes, dietary changes, environmental factors, taking drugs, strenuous exercise and menstrual period increase fibrinolytic activity, high-fat food can increases blood lipid and inhibits fibrinolytic activity. What's more, smoking can increase platelet aggregation,drinking water can inhibit the aggregation. For the oral contraceptives, it can increase coagulation activity and reduce fibrinolytic activity.
