New Arrival China Conical Bottom Tube - Single Muclear Cell Gel Separation Tube—CPT Tube – Lingen

Monocyte preparation tube (CPT tube) has added the design of built-in Ficoll – Hypaque, anticoagulant and separation gel. Lymphocytes and mononuclear cells can be easily isolated from whole blood by one-step centrifugation using a special cell separation gel. It is mainly used for lymphocyte immune function detection, HLA or residual leukemia gene detection and immune cell therapy. It provides a standardized method for one-step extraction of monocytes for clinical diagnostic specimen preparation and cellular immunotherapy.

1) Size: 13*100mm, 16*125mm;

2) Additive Volume: 0.1ml, 135usp;

3) Blood Volume: 4ml,8ml;

4) Shelf Life: 24 months from the date of manufacture;

5) Storage: Store at 18-25 ℃.

1) Efficient, accurate and safe;

2) With built-in Ficoll Hypaque, anticoagulant and separation gel, mononuclear cells are separated from whole blood by one-step centrifugation.

3) Precise cell separation technology.

4) Inner wall bionic membrane processing technology;

5) The recovery rate of monocytes is more than 90%, the purity is more than 95%, and the survival rate is more than 99% 

Several problems needing attention in cell separation:

1) If you want to do cell culture, remember that the reagents (separation solution, washing solution, etc.) and instruments used in the experiment should be sterile, and pay attention to the aseptic operation in the experiment.

2) Centrifugation temperature: some require room temperature; others require 4 degrees Celsius. The operation of the experiment should be as skilled and coherent as possible.

3) When separating mononuclear cells (PBMC) with Ficoll, it usually contains a small amount of red blood cells, which has little impact on the general experiment. If the experimental requirements are high, lysate can be used (some need to be sterile), and pay attention to the control of lysis time to avoid affecting the activity of mononuclear cells.

4) The effect of centrifugation with whole blood is worse than that of re centrifugation after whole blood dilution. The dilution is generally double the equal volume dilution.