Background: The development of platelet-rich fibrin (PRF) drastically simplified the preparation procedure of platelet-concentrated biomaterials, such as platelet-rich plasma (PRP), and facilitated their clinical application. PRF’s clinical effectiveness has often been demonstrated in pre-clinical and clinical studies; however, it is still controversial whether growth factors are significantly concentrated in PRF preparations to facilitate wound healing and tissue regeneration. To address this matter, we performed a comparative study of growth factor contents in PRP and its derivatives, such as advanced PRF (A-PRF) and concentrated growth factors (CGF).
Methods: PRP and its derivatives were prepared from the same peripheral blood samples collected from healthy donors. A-PRF and CGF preparations were homogenized and centrifuged to produce extracts. Platelet and white blood cell counts in A-PRF and CGF preparations were determined by subtracting those counts in red blood cell fractions, supernatant acellular serum fractions, and A-PRF/CGF exudate fractions from those counts of whole blood samples. Concentrations of growth factors (TGF-β1, PDGF-BB, VEGF) and pro-inflammatory cytokines (IL-1β, IL-6) were determined using ELISA kits.
Results: Compared to PRP preparations,both A-PRF and CGF extracts contained compatible or higher levels of platelets and platelet-derived growth factors. In a cell proliferation assay, both A-PRF and CGF extracts significantly stimulated the proliferation of human periosteal cells without significant reduction at higher doses.
Conclusions: These data clearly demonstrate that both A-PRF and CGF preparations contain significant amounts of growth factors capable of stimulating periosteal cell proliferation, suggesting that A-PRF and CGF preparations function not only as a scaffolding material but also as a reservoir to deliver certain growth factors at the site of application.
Keywords: Growth factor, Platelet-rich plasma, Platelet-rich fibrin, Plasma rich in growth factors, Concentrated growth factors Abbreviations: ACD, Acid citrate dextrose solution; ANOVA, Analysis of variance; A-PRF, Advanced platelet-rich fibrin; A-PRFext, A-PRF extract; CGF, Concentrated growth factors; CGFext, CGF extract; ELISA, Enzyme-linked immunosorbent assay; IL-1β, Interleukin-1β; IL-6, Interleukin-6; PDGF-BB, Platelet-derived growth factor-BB; PLT, Platelet; PRGF, Plasma rich in growth factors; PRP, Platelet-rich plasma; RBC, Red.
Post time: Oct-12-2022